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These techniques revealed a bi-phasic interaction mode consisting of high-affinity DNA binding characterised by stabilisation of duplex DNA, followed by molecular condensation associated with charge neutralisation

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[DOI] [PubMed] [Google Scholar] [149].Zhang WJ, Frei B

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Applying several hours before bed is fine but slightly suboptimal

You want to understand what protocols are being used in real clinics, what the safety boundaries are, and how to handle this without putting your license or your patients at risk